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Image Search Results
Journal: STAR Protocols
Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation
doi: 10.1016/j.xpro.2022.101532
Figure Lengend Snippet: Schematic of the closed-loop neural recording setup for sleep/wake response tracking Video recording, stimulation, and LED illumination are controlled using an Arduino microcontroller. Chemical stimulation through the microfluidic arena switches between stimulus (S) and buffer (B) solutions via control fluid flow (C). Brightfield images are used to track sleep behavior and fluorescent images are used to measure GCaMP calcium transients in neurons. Image capture, sleep/wake state determination, and chemical stimulation are controlled by a computer in a closed loop, without user intervention.
Article Snippet:
Techniques: Control
Journal: STAR Protocols
Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation
doi: 10.1016/j.xpro.2022.101532
Figure Lengend Snippet: Sample experiment settings file These sample settings define imaging resolution (bin 2), framerate (100 ms exposure = 10 frames/s), and illumination timing (20 ms for brightfield, 10 ms for fluorescence with 5 ms delay from exposure onset). Stimulation recordings capture 300 frames (or 30 s at 10 fps), and the stimulation program is a 10 s chemical pulse (valve 1 opens from frame 50–150 or from 5–15 s within each recording trial). The entire experiment lasts 12 h. Sleep state changes are monitored using a 10-s interval and changes must persist for 3 intervals (i.e., 30 s) to register a transition. After each stimulation trial, 15 min should elapse before triggering a new stimulation (regardless of any transitions within this time delay), and the next trigger shall begin following the opposite transition (i.e., Sleep-to-wake follows Wake-to-sleep).
Article Snippet:
Techniques: Imaging, Fluorescence
Journal: STAR Protocols
Article Title: Monitoring neural activity during sleep/wake events in adult C. elegans by automated sleep detection and stimulation
doi: 10.1016/j.xpro.2022.101532
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Expressing, Recombinant, Software, Control, Isolation, Fluorescence, Inverted Microscopy